Figure 4. Neutrophils heterozygous for the Mcl-1 allele are more sensitive to CDK inhibition.
A. SNS-032 and PIK75 induce Bak/Bax-dependent apoptosis in primary murine bone marrow neutrophils that is sensitized by haplo-insufficiency of Mcl-1. Dose-dependent changes to neutrophil viability induced by PIK-75 or SNS-032 were assessed by live cell imaging over 20 hours, with genotypes and concentrations as marked. EC50 values were calculated from the 12 hour time-point, data represent mean ± SEM with n = 3 per genotype. B. Immunoblot of Mcl-1 protein in bone marrow neutrophils from Mcl-1+/- mice, and from mouse embryonic fibroblasts as a control.