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. 2017 May 25;8(35):58003–58020. doi: 10.18632/oncotarget.18217

Figure 1. Development of the TSf fragment.

Figure 1

A. The TSf fragment obtained showed 90% aminoacidic identity as aligned with the TS indexed in the GenBank AJ276679 [15]. B. A representative picture of the TSf fragment as compared to the full-length TS protein. C. Assessment of TSf protein purification on SDS polyacrylamide gel electrophoresis: lane 1 non-induced colony, lane 2: TSf protein that was purified in Ni-NTA resin-based affinity chromatography and dialyzed against urea 0,5M; lane 3: TSf protein expressed but not purified, lane 4: low molecular weight marker.