Figure 3. UBC cells produce and secrete exosomes with a specific miRNA expression pattern depending on the invasiveness of parental cells.

(A) The miRNA expression levels in exosomes secreted by invasive UBC cells (blue) compared to exosomes obtained from non-invasive UBC cells (yellow). Unsupervised hierarchical cluster analysis of differentially expressed miRNAs. P-value was determined using Mann–Whitney U test. (Biological replicates n = 2 per cell type; p-value < 0.05; Fold Change > 1.5; σ = 0.2); (B) Expression level of 5 deregulated miRNAs in exosomes secreted by invasive UBC cells (grey) compared to exosomes from non-invasive cells (dark blue). P-value was determined using Mann–Whitney U test. (Biological replicates n = 3 per cell line; invasive cells n = 9; non-invasive cells n = 6); P = p-value; FC = Fold Change.