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. 2017 Apr 6;8(35):58338–58352. doi: 10.18632/oncotarget.16873

Figure 3. TNF-α activates HBXIP promoter through activating transcription factor STAT3.

Figure 3

(A) The illustration of wild or mutant sequence responsive to STAT3 in HBXIP promoter was shown. (B) The relative activities of HBXIP promoter containing wild type (wt) or mutant type of STAT3 (STAT3 mut) binding site were detected by luciferase reporter gene assays in MDA-MB-468 cells administrated with 10 ng/ml, 20 ng/ml TNF-α. (C) The relative activities of the promoter of HBXIP were detected by luciferase reporter gene assays in MDA-MB-468 cells administrated 20 ng/ml TNF-α and transiently transfected with STAT3 siRNA. (D) The enrichment of transcription factor STAT3 in the HBXIP promoter was detected by CHIP assays by using antibody against p-STAT3 in MDA-MB-468 cells upon TNF-α treatment. (E) The levels of STAT3, p-STAT3 were detected by Western blot analysis in MDA-MB-468 cells transfected with STAT3 siRNAs. Error bars represent ±s.d., *p< 0.05, **p< 0.01, ***p< 0.001, Student's t test. All experiments were repeated at least 3 times.