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. 2017 Apr 11;8(35):58480–58493. doi: 10.18632/oncotarget.17024

Figure 2. Alteration of bone metastatic and / or bone-related gene expression in osseous metastases.

Figure 2

(A) Representative images of protein expression by western blotting (n = 3 for each group). (B) Quantitative results of protein expression were made by two image analysis experts using Image J software (National Institutes of Health, Bethesda, MD, USA) (log2-ratio vs. vehicle; mean ± SD). * p < 0.05 vs. vehicle. (C) Quantitative results of mRNA expression determined with RT-qPCR. RT-qPCR measurements (log2-ratio vs. vehicle; mean ± SD) in 6 individual mRNA samples obtained from bone metastasis lesions with or without osthole treatment. * p < 0.05 vs. vehicle. Mice were sacrificed at the end of treatment, and metastatic bone lesions were harvested and stored in liquid nitrogen. Frozen tissue was homogenized with a pestle and total RNA and protein were extracted for RT-qPCR and western blotting, respectively.