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. 2017 Apr 19;8(35):58536–58552. doi: 10.18632/oncotarget.17234

Figure 2. High expression of mitochondrial GRP75 in G2/M-phases.

Figure 2

(A) Predication of expression changes of HSP7M (GRP75 homologue), Hsc70, TfnR, clathrin, and caveolin-1/-2 in sub-phases of cell cycle usingCyclebase 3.0 analysis (dark blue content indexes the expression level of mRNA/protein during the cell cycle); (B) Western blot determined the expression level of those mentioned above in synchronized HeLa subcellular fractions. lysate-: whole cell lysate, memb-: membrane lysate, and mito-: mitochordrian lysate. Protein brands were quantified by Image J software and the expression ratio (compared to that of non-treated cells, Ctrl, set as 1) in the sub-phase is correspondingly marked below. Mitotic (arrow pointing) and interphase HeLa cells were stained by concentration-increased MTK-077 dye (C), or co-stained by anti-GRP75 antibody (1:100) together with the mitochondrial marker mitoTracker®Red (300nM) (D). Similar results were found in Cos-7 cells (Data not shown).