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. 2017 Apr 24;8(35):58611–58624. doi: 10.18632/oncotarget.17381

Figure 3. PPA1 promotes proliferation in colon cancer cell lines.

Figure 3

(A) Western Bolt results showed that PPA1 was higher expressed in colon cancer cell lines than that in normal epithelial CCD-18Co cells. SW480 cells showed highest PPA1 expression while HT29 showed lowest PPA1 expression. (B) Upon PPA1-silencing, the pJNK level was significantly increased without changes in total JNK protein level. Meanwhile, PPA1 knock-down increased Bax and p53 accumulation, Bcl-2 phosphorylation as well as Caspase-3 cleavage. In contrast, overexpression of PPA1 showed reciprocal regulation towards these signaling proteins. No significant change of pERK or p-p38 levels was observed. Knock-down of PPA1 in SW480 cells inhibited cell proliferation (C), while overexpression of PPA1 in HT29 cells showed opposite effect (D). Data presented were results from three repeated experiments.