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. 2017 Apr 24;8(35):58611–58624. doi: 10.18632/oncotarget.17381

Figure 5. Purification and enzymatic characterization of PPA1.

Figure 5

(A) SDS-PAGE gels with Coomassie staining showed the high purity of PPA1-WT, PPA1-D117A and JNK1 proteins. (B) The synthesized phosphor-peptides and corresponding phosphor-sites were listed. (C) PPA1 can dephosphorylate pJNK phosphor-peptides (reaction time was 10min), while its inactive mutant PPA1-D117A (reaction time was 90min) almost abolished its activity. (D) PPA1 can directly dephosphorylate pJNK1 protein which was generated by in vitro methods. Data presented were results from three repeated experiments.