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. 2017 Apr 24;8(35):58625–58641. doi: 10.18632/oncotarget.17382

Figure 6. BMPR2 activates ROCK/LIMK signaling through a Smad-independent pathway.

Figure 6

(A) Cytoskeletal assay of 143B and U2OS cells was visualized by confocal microscopy. Representative images were shown. Cell nuclei were stained with Hoechst33342. Scale bar represents 25 μm or 10 μm. (B) BMPR2-silencing attenuates p-LIMK expression through the Smad-independent pathway. (C) Smad1 and Smad5 are not required for BMPR2-depleted down-regulation of p-LIMK2. (D) GTPase activation assay validated the changes of RhoA in U2OS cells. (E) Interaction of LIMK2 with BMPR2 in U2OS cells. U2OS cells were transfected with Flag-BMPR2 and Flag-control. Cell lysates were immunoprecipitated with Flag-beads, and immunoblotted with anti-BMPR2 (top panel) or anti-LIMK2 antibodies (bottom panel).