Figure 3. Negative correlation between HOTAIR and SAV1.

(A) HOTAIR expression was negatively correlated with SAV1 at the mRNA level in RCC clinical samples. (B) SAV1 protein was downregulated in cancer tissues with high HOTAIR expression levels. (C and D) HOTAIR knockdown significantly downregulated SAV1 mRNA and protein, whereas HOTAIR overexpression upregulated SAV1. Hippo pathway proteins were detected with a Hippo Pathway Antibody Kit (cat:8579, Cell Signal Technology, Beverly, MA, USA). SAV1, large tumor suppressor homolog (LATS)-1, LATS2, phosphorylated MOB, Yes-associated protein 1 (YAP1), and phosphorylated YAP1 levels were significantly altered by HOTAIR knockdown or overexpression.