Figure 2. Activation of other RTKs in acquired resistance to ALK inhibitors.

(A) Cells were grown to confluence, and cell lysates were then prepared by protein extraction. 500 μg of the cell lysates were incubated on each membrane. A phospho-RTK array was performed as described in the Materials and Methods. (B) Lysates from each cell line were subjected to Western blot analysis. The indicated antibodies were used to evaluate the level of activated protein in Figure 2A.