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. 2017 Jul 19;8(35):58865–58871. doi: 10.18632/oncotarget.19378

Figure 4. chZAP interacted with SU of ALV-J in cytoplasm.

Figure 4

(A) ALV-J and endogenous chZAP are both present in cytoplasm of DF-1 cells. At 48 h post infection with ALV-J, the protein expression level of chZAP of infected cells was very significantly higher than the uninfected normal control cells. The merged yellow fluorescence indicated the interaction between chZAP and ALV-J. (B) chZAP interacted with SU. DF-1 cells were transfected with empty vector (chZAP-) or LV-chZAP expressing flag-chZAP (chZAP+). The cells were harvested at 72 h post infection with ALV-J. Lysates were immunoprecipitated with anti-Flag antibody and detected by western blotting using anti-SU antibody.