Figure 4. Proliferative effect of mast cells on BPH-1 cells.

(A) BPH-1 cells were cultured alone or co-cultured with mast cells and assayed with MTT at 72 hours. (B) Cell-cycle stage were assessed using PI staining and flow cytometry in BPH-1 cells with or without co-culturing with mast cells. Graphs shown are mean of three experiments. *P < 0.05 by Student's t-test.