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. 2017 Jul 22;8(35):59246–59256. doi: 10.18632/oncotarget.19495

Figure 5. KLF4 is a direct target of miR-543 and KLF4 expression is inversely correlated with miR-543 expression in CRC tissues.

Figure 5

(A) Western blot analysis of KLF4 protein expression after transfection in HCT116 and SW-480 cells. (B) Luciferase activities of wild-type and the mutant pmirGLO-KLF4-3′-UTR reporter in HCT116 and SW-480 cells. (C) The predicted miR-543 binding site on the KLF4 mRNA 3′-UTR and the corresponding mutations in 3′-UTR of KLF4. (D, E) KLF4 expression on protein level (D) and mRNA level (E) was determined in CRC tissues (T) and adjacent nontumorous tissues (ANT). (F) Spearman's correlation analysis was performed to detect the association between the expression level of miR-543 and KLF4 in GC tissues. (G, H) KLF4 expression on protein level (G) and mRNA level (H) was determined in three CRC cell lines (HCT116, SW-480 and HT29) and nontumorous mucosa. Error bars represent mean ± SD from three independent experiments. * p < 0.05.