Figure 5. Nude mice (6 per group) were inoculated subcutaneously with 3×106 A549 cells and treated i.p. 500 nM /dose 5-Aza-CR or 10 nM siRNA-DNMT1 or negative control.

The tumor volume (mm³) (A-B) and body weight (C) were measured. After sacrifice, the tumor tissues were collected to analyze the expression (D) and the status of methylation of RASSF1A and APC (E-G). *P< 0.05, **P< 0.01, ***P< 0.001. Data represent the means ± s.d.