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. 2017 Jul 31;8(35):59435–59445. doi: 10.18632/oncotarget.19738

Figure 2. Effects of LL22NC03-N64E9.1 on CRC proliferation and apoptosis in vitro.

Figure 2

(A) Analysis of LL22NC03-N64E9.1 expression levels in CRC cell lines (DLD-1, Lovo, HT-29, SW620, HCT116 and SW480) by qRT-PCR. (B) LL22NC03-N64E9.1 knockdown in CRC cells transfected with siRNA. (C) Representative results of the colony formation of SW480, DLD-1 and Lovo cells transfected with the siRNA of LL22NC03-N64E9.1. (D) MTT assays were performed to determine the cell viability of CRC cells after the transfection of siRNA against LL22NC03-N64E9.1. (E) Flow cytometry assays were performed to analysis the cell cycle progression when SW480 and DLD-1 cells transfected with siRNA against LL22NC03-N64E9.1. (F) Flow cytometry assays were performed to analysis the cell apoptotic in siRNA-transfected SW480 and DLD-1 cells. Representative images and data based on three independent experiments. Bars: s.d, *P<0.05, **P<0.01.