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. 2017 Jul 31;8(35):59435–59445. doi: 10.18632/oncotarget.19738

Figure 5. LL22NC03-N64E9.1 negatively regulates expression of KLF2 by rescue experiments.

Figure 5

(A) The levels of KLF2 mRNA expression were determined by qRT-PCR when SW480 cells were transfected with si-NC, si-KLF2 1#, si-KLF2 1# and Empty vector, pcDNA-KLF2. (B) MTT assays were performed to determine the cell viability of SW480 cells after the transfection of siRNA against KLF2. (C) MTT assays were performed to determine the viability of pcDNA-KLF2-transfected SW480 cells. (D, E) MTT and colony formation assays were used to determine the cell proliferation ability for SW480 cells transfected with si-NC and siRNA 1# and co-transfected with siRNA 1# and si-KLF2. (F) Flow cytometry assays were performed to analyze the cell apoptosis when SW480 cells were transfected with si-NC and siRNA 1# and co-transfected with siRNA 1# and si-KLF2. Representative images and data based on three independent experiments. Bars: s.d, *P<0.05, **P<0.01.