Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 15;8:554. doi: 10.1038/s41467-017-00694-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — PolD1 associates with γTuRCs. a Anti-GCP6 immunoprecipitation was performed using HEK293T cell extracts; normal rabbit IgG was used in the control immunoprecipitation. The immunoprecipitates were immunoblotted with the indicated antibodies. b HEK293T cells were transfected with control or pold1-targeting siRNAs, and then immunoprecipitation was performed using normal rabbit IgG or anti-GCP4 antibody. c FLAG-PolD1 was expressed in cells that were transfected with a GCP4-specific siRNA (or a control siRNA), and then anti-FLAG immunoprecipitation was performed. The immunoprecipitates and the lysate inputs were immunoblotted with the indicated antibodies. FLAG-PolD1 was detected through anti-FLAG immunoblotting. d Extracts of HEK293T cells ectopically expressing PolD1 or its mutants (D515V and S605del) were used for anti-FLAG immunoprecipitation