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. 2017 Sep 15;7:11710. doi: 10.1038/s41598-017-12188-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic overview of continuous exchange cell-free (CECF) protein synthesis based on CHO cell lysates. A two chamber reaction device (RiNA GmbH) serves as a basis for CECF protein production (upper part of the figure). The reaction and a feeding chamber are separated by a 10 kDa cutoff, semipermeable membrane enabling a semi continuous delivery of energy to the reaction chamber and a removal of inhibitory byproducts.