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. 2017 Sep 11;8:1698. doi: 10.3389/fmicb.2017.01698

Figure 2.

Figure 2

Prevention of Cre-mediated plasmid recombination. (A) Schematic map of the integration of uORF and lacO. The sequences of uORF and lacO are underlined; the downstream Xho I sites are in bold. (B) Single-colony PCR analysis. Line M, DNA marker; Line 1, the detected fragment of Cre-AOX1TT-PTEF1-PEM7-ZeoR-CYC1TT-lox66; Line 2, the detected fragment between lox71 and lox66 of pMCO; Line 3, the detected fragment between lox71 and lox66 of pMCU. (C) Gel electrophoresis analysis of extracted recombinant plasmids from E. coli. Line M, DNA marker; Line 1, pMCO; Line 2, pMCU. (D) RT-PCR analysis of E. coli (pMCO). Line M, DNA marker; Line 1, positive control of 16s rRNA gene; Line 2, cre gene.