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. 2017 Sep 11;8:1698. doi: 10.3389/fmicb.2017.01698

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Copyright © 2017 Li, Zhang, Li, Zhou, Cao, Huang and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Scheme for construction and identification of the expression vectors with 1, 2, 4, or 8 copies of the appA gene. (A) Schematic map of the construction of expression vectors pMCO-AOXα-A1, pMCO-AOXα-A2, pMCO-AOXα-A4, and pMCO-AOXα-A8. (B) Gel electrophoresis analysis of recombinant vectors digested with Spe I and Xba I. Line M, DNA marker; Line 1, pMCO-AOXα-A1; Line 2, pMCO-AOXα-A2; Line 3, pMCO-AOXα-A4; Line 4, pMCO-AOXα-A8.