Figure 5.

Identification and expression analysis of the transformants that were resistant or sensitive to Zeocin. (A) Expression analysis of the Zeocin^R transformants. (B) Identification of the colonies that were sensitive toward Zeocin. (C) Single-colony PCR analysis of Zeocin^S strains using primer pairs lox-F/αf-R. Line M, DNA marker; Line 1, Zeocin^R transformants; Line 2-11, 10 randomly selected Zeocin^S transformants. (D) Expression analysis of the Zeocin^S transformants. The experiments were performed 10 or 20 times as described; the mean values ± SD are presented. Bars with the same letters are not significant (P < 0.05).