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. 2017 Sep 11;8:1698. doi: 10.3389/fmicb.2017.01698

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Copyright © 2017 Li, Zhang, Li, Zhou, Cao, Huang and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression analysis of the Zeocin^S transformants. (A) SDS-PAGE analysis. Line M: protein marker; Line 1: GS115-pMCO-AOXα-A1-Zeocin^S #2; Line 2: GS115-pMCO-AOXα-A2-Zeocin^S #3; Line 3: GS115-pMCO-AOXα-A4-Zeocin^S #1; Line 4: GS115-pMCO-AOXα-A8-Zeocin^S #17; Line 5: GS115-pMCO-AOXα-A8A4-Zeocin^S #5; Line 6: GS115-pMCO-AOXα-A8A8-Zeocin^S #7. (B) Production of phytase with different copy number of the appA gene. The experiments were performed 3 times; the mean values ± SD are presented. Bars with the same letters are not significant (P < 0.05).