Table 4.
The phytase yield and the copy number analysis of the ZeocinS transformants.
| Strains | Exhibited highest yield of phytase (U/mL) | Improvement of yield of recombinant protein (fold) | Copy number of appAa |
|---|---|---|---|
| GS115-pMCO-AOXα-A1-ZeocinS #2 | 95.11 | 1.00 | 1.00 |
| GS115-pMCO-AOXα-A2-ZeocinS #3 | 153.43 | 1.61 | 1.82 ± 0.06 |
| GS115-pMCO-AOXα-A4-ZeocinS #1 | 240.73 | 2.53 | 3.49 ± 0.13 |
| GS115-pMCO-AOXα-A8-ZeocinS #17 | 358.38 | 3.77 | 7.34 ± 0.65 |
| GS115-pMCO-AOXα-A8A4-ZeocinS #5 | 417.79 | 4.39 | 11.08 ± 0.51 |
| GS115-pMCO-AOXα-A8A8-ZeocinS #7 | 371.52 | 3.91 | 14.75 ± 0.29 |
a
The copy number of appA in the calibrator strain harboring plasmid pMCO-AOXα-A1 was defined as 1 copy.