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. 2017 Sep 15;8:558. doi: 10.1038/s41467-017-00632-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Combining a systematic suppressor screen with mRNA abundance data to identify P-body targets in replication stress. a Overview of the screen to identify suppressors of the HU sensitivity of P-body mutants. b Z-score normalized HU fitness values for the lsm1∆ xxx and pat1∆ xxx double mutants. Genes that were identified in both screens are indicated in green. Validated hits are indicated on the right. c The mRNA abundance during replication stress is displayed as a heatmap for eleven candidate genes from the intersection of the suppressor screen and mRNA abundance data. The log2 lsm1∆:WT ratio is plotted for the indicated time points. d HU sensitivity assay for the 6 validated candidate genes among the 11 genes identified in c. Ten-fold dilutions of an overnight culture were spotted on YPD medium with or without HU. e Biological functions of the validated candidate genes