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. 2017 Sep 15;7:11732. doi: 10.1038/s41598-017-11992-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) H&E stained trachea sections from the normal and injury groups for direct and indirect co-culture assays: (left panel) Normal group with intact pseudostratified epithelium, and (right panel) injury group following brushing-induced tracheal injury. Insets show the epithelium layer at higher magnification. (b) Brushing technique removes tracheal epithelium layer. (c) H&E stained cell collection from tracheal brushing, which predominantly consists of ciliated cells (scale bars (a) = 20 μm, (c) = 50 μm).