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. 2017 Sep 15;7:11732. doi: 10.1038/s41598-017-11992-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AEC morphology and characterisation. (a) Phase-contrast micrographs of confluent monolayer of AECs with cobblestone-like appearance. Representative images of DAPI (blue) and (b) BrdU (green) as well as (c) epithelial cell marker K5+8 (green), K14 (green), and tubulin (green) staining of AECs following isolation and culture. The detection of BrdU-labelled cells on the trachea sections following direct co-culture assay. (d) H&E stained trachea sections of anti-BrdU staining (green) following direct co-culture assay (d1 = day 1, d5 = day 5). Arrows indicate BrdU+ cells that formed the lining of epithelium. Insets show the cell(s) at higher magnification (scale bars (a) = 100 μm, (b, c, d) = 50 μm, scale bars of insets represent 10 μm).