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. 2017 Sep 15;7:11734. doi: 10.1038/s41598-017-11967-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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“lux box 2” is required for repression of ainSR promoter-reporter by LuxR-3OC6-HSL. Fluorescence of cells harboring P_ainS -gfp reporter pHK12 grown in SWTO to OD₅₉₅ ~2.5. Strains with luxR ^ES114 or no luxR harboring P_ainS -gfp reporter plasmids pHK156 (with lux box 1) or pHK12 (both lux boxes) were grown with 50 nM 3OC6-HSL (white bars) or no addition (grey bars). Strains harboring these reporters lack AHL synthases (∆luxI ∆ainS) and feedback regulation of luxR (litR::ermR P_con -luxR). Bars indicate standard error (n = 3), and asterisk indicates a significant difference in reporter activity upon addition of 50 nM 3OC6-HSL (P < 0.05).