Figure 3.

Ex vivo immunological studies of wild-type (WT) and C4 KO mice in experimental autoimmune uveitis (EAU). At day 21 after EAU induction, splenocytes were collected from WT and C4 KO mice and cultured for 72 h without antigen (w/o) or with 20 µg/ml of the immunizing interphotoreceptor retinoid binding protein (IRBP) peptide or a non-relevant peptide (OVA), then, levels of interferon (IFN)γ (A) and IL-17 (B) were measured by ELISA (n = 22 in each group) mean ± SEM; *p < 0.05 by one-way ANOVA. On the same day, eyes were collected from five mice in each group and single cell suspensions prepared and pooled, then the percentage of CD4+ T cells in the infiltrating cells in the eye of WT (C) and C4 KO (D) mice was analyzed by flow cytometry.