Figure 4.

Effects of C4 deficiency on DC differentiation and function. BM cells from naïve wild-type (WT) and C4 KO mice were cultured under DC differentiation conditions and differentiated double-positive cells (DCs) (MHCII+ CD11C+) analyzed by flow cytometry. (A,B) Representative flow cytometric analysis results for WT and C4 KO DC differentiation (A) and the summarized results (B) [n = 5 in each group, mean ± SEM]. (C,D) The same numbers of BM-derived WT or C4 KO DCs was then cultured with carboxyfluorescein succinimidyl ester (CFSE)-labeled T cells from OTII mice in the presence or absence (wo) of peptide OVA_323–339 for 72 h, then the proliferation of the activated T cells was assessed by flow cytometry (C) and levels of interferon (IFN)γ produced by the activated T cells measured by ELISA (D) (n = 5 in each group). The same T cell proliferation (E) and IFNγ (F) assays were done using splenic DCs isolated from WT and C4 KO mice (n = 3 in each group), no statistically significant difference was observed either (mean ± SEM).