Fig. 3.

—Tsetse sex-specific differences in Wigglesworthia expression. (A) Principal components analysis (PCA) of Wigglesworthia expression within male and female bacteriomes. Each point represents the Wigglesworthia transcriptomic signature of one RNA-Seq library. The directionality of separation was observed in the Principal Component (PC) 2, with 24% variance. 95% confidence circles are indicated. (B) A small number of differentially expressed Wigglesworthia genes were found between female and male bacteriomes. A consensus of 4 Wigglesworthia loci was found to be significantly higher expressed in female bacteriome libraries utilizing different analytical methods; DESeq (Differential expression of RNA-Seq data), Kall–Sleuth (Kallisto–Sleuth), and R_ANOVA (R statistical package, Analysis of variance). (C) Histogram showing the distribution of fold diff (avg. TPM) across the Wigglesworthia genome for compiled RNA-Seq libraries. The Wigglesworthia genome is depicted linearized, colored circles are associated with specific functional groups, the red dashed line indicates 1.5 fold diff. (avg. TPM).