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. 2017 Sep 11;8:1122. doi: 10.3389/fimmu.2017.01122

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Copyright © 2017 Wu, Zhang, Yin, Fu, Jiang, Ding, Chu, Shang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miR-21 identified as potential regulator of interferon-inducible protein 10 (IP-10) secretion. miRNA candidates were screened, using three target-prediction software programs, including TargetScan, miRanda, and DIANA for binding sites. (A) miRNAs and IP-10 3′UTR matching sequences; (B) IP-10 mRNA expression exhibited by B cells, monocytes, CD4⁺ and CD8⁺ T cells, and NK cells; (C) THP-1 cells were transfected with either mimics of six miRNAs or negative control for 48 h and stimulated by lipopolysaccharide (LPS) for 24 h. IP-10 production in supernatants of THP-1 cells was measured.