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. 2017 Sep 15;6:e29236. doi: 10.7554/eLife.29236

Figure 1. (A) Size exclusion chromatography elution traces of recombinantly expressed NSs variants, detected by UV absorption at 280 nm.

Elution volumes for molecular mass markers used for calibration of the column are indicated with dashed lines. (B) 1H-15N HSQC NMR spectra of NSs-ΔN (blue) and NSs-ΔNΔC (red) overlaid. The right-hand panel shows the central region of the spectra plotted at high contour levels, selecting a subset of intense crosspeaks representing highly dynamic amino acid residues, most of which are not present in NSs-ΔNΔC. The dashed line connects crosspeaks of one side-chain amide.

Figure 1.

Figure 1—figure supplement 1. CD spectra of NSs.

Figure 1—figure supplement 1.

(A) Far-UV CD spectrum of FL-NSs. (B) Far-UV CD spectrum of NSs-ΔN. (C) Near-UV CD of FL-NSs shows a weak but distinctive signal (blue) as compared with buffer (green), indicating presence of tertiary structure. (D) Near-UV CD of NSs-ΔN (blue), NSs-ΔNΔC (maroon) and buffer (green). Matching minima and maxima observed in spectra of all three NSs variants are indicated by colored arrows.