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. 2017 Sep 4;6:e24994. doi: 10.7554/eLife.24994

Figure 4. COPS7B influences PER2 expression and cellular clock function.

(a) Expression levels of PER2 stratified by rs920400 genotype. (AA vs. GG genotype, T-test p=0.00026). Insets - Correlation between PER2 and COPS7B gene expression within each genotype. (b) Reduction of PER2 expression (grey) and COPS7B expression (black) in HEK293T and U2OS cells transfected with three short interfering RNAs targeting COPS7B (c) Raw bioluminescence profiles of U2OS cells cotransfected with a Bmal-luciferase circadian reporter and siRNAs targeting COPS7B (dotted lines) or scrambled siRNA (solid lines). (d) Reduction in circadian amplitude by short interfering RNAs targeting COPS7B (p=0.0021, student T-test). These siRNAs also lengthen the circadian period by 1.15 hr (p=3.27094E−05). See also Figure 4—figure supplement 1 for similar RNAi assays performed upon the COPS4 gene.

Figure 4.

Figure 4—figure supplement 1. Effects of COPS4 depletion upon circadian period and amplitude.

Figure 4—figure supplement 1.

(a) Raw bioluminescence profiles of U2OS cells cotransfected with a Bmal-luciferase circadian reporter and siRNAs targeting COPS4 (dotted lines) or scrambled siRNA (solid lines). (d) Reduction in circadian amplitude by short interfering RNAs targeting COPS4 (p=6.47781E−07, student T-test).