Table 2.

The summary of the most significant technical details related to immunohistochemical staining

Type of antibody	Type/clone	Diluent type	Primary antibody dilution	Incubation time (min)	Positive control
iNOS Novus Biologicals, UK	Polyclonal	Antibody Diluent with Background Reducing Components Dako, Denmark	1:300	30	Appendix
FoxP3 Zytomed Systems, Germany	Monoclonal SP97	EnVisionTMFLEX Antibody Diluent Dako, Denmark	1:150	30	Tonsil
CD8 Dako, Denmark	Monoclonal C8/144B	EnVisionTMFLEX Antibody Diluent Dako, Denmark	1:1	20	Tonsil
CD68 Dako, Denmark	Monoclonal PG-M1	EnVisionTMFLEX Antibody Diluent Dako, Denmark	1:100	20	Tonsil