Table 1.

Parameters used for quantification and statistical analysis.

Type and analysis	Cochleas	Cochleas total	Analysis area (µm)	Measurements/sample	Measurements total	n	Average	SD	Two-tailed t test
Reticular lamina length (µm)
E18									1.17 × 10–11
Control		4				18	18.44	3.94
SBEM	1		70	9	9
Confocal	3		3 × 100	3	9
Gfi1GFP/GFP		3				12	5.47	0.84
SBEM	1		61	3	3
Confocal	2		2 × 100	3	9				0.051
Adult
Control		4				13	25.58	2.02
SBEM	2		46; 75	3; 4	7
Confocal	2		2 × 100	3	6
KAFU		4				12	24.04	2.04
SBEM	2		67; 75	3	6
Confocal	2		2 × 100	3	6
Deiters cell surface area (µm2)
E18									0.41
Control		3				46	13.08	3
SBEM	1		70	25	25
Confocal	2		2 × 100	9;12	21
Gfi1GFP/GFP		3				55	12.77	4.15
SBEM	1		61	27	27
Confocal	2		2 × 100	9;19	28
Adult									1.23 × 10–27
Control		3				46	25.23	5.32
SBEM	2		46; 75	10;16	26
Confocal	1		100	20	20
KAFU		4				60	43.97	7.55
SBEM	2		67; 75	6;14	20
Confocal	2		2 × 100	18;22	40
Hair cell surface area (µm2)
OHCs									1.90E-22
E18 control
Confocal	6	50–120	19; 15; 17; 19; 31; 57	158	28.91	3.21
E18, TM E13–14 RhoAfl/fl;Fgfr3-iCreERT2
Confocal	7	50–120	10; 17; 14; 21; 7; 25; 33	127	45.62	15.72
IHCs									0.61
E18 control
Confocal	6	50–120	6; 5; 5; 6; 8; 19	49	36.1	4.81
E18, TM E13–14 RhoAfl/fl;Fgfr3-iCreERT2
Confocal	5	50–120	5; 6; 10; 10; 11	42	35.5	5.94