FIGURE 1.

Principle of transcriptome-wide m⁶A profiling using m⁶A-seq. RNA is fragmented and fragments containing methylated adenosines immunoprecipitated using an anti-m⁶A antibody. Fragments are sequenced together with a matched RNA-seq control and all sequenced reads are aligned to the reference transcriptome. Reads from the immunoprecipitated experiment pile up into distinct peaks that show enrichment over RNA-seq control, indicating the presence of a methylated adenosine residue.