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. 2017 Oct;23(10):1502–1511. doi: 10.1261/rna.056408.116

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© 2017 Niland et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — Variation of nucleotides in the 5′ leader of pre-tRNA contacting the P RNA subunit of RNase P alters the energetic contribution of nucleotides contacting the C5 protein. (A) The 16 data subsets of HTS-Kin separated by the identity of nucleotides at N(−2) and N(−1) in the RNA binding site. (B) Each subset is compared to the genomically encoded A(−2)U(−1). Each row symbolizes a change in nucleotide identity at the N(−1) position while each column follows a change at N(−2). Each of the 16 boxes represents a scatter plot of the k_rel for a substrate with a given sequence at N(−6) to N(−3) in the background of the mutant or wild-type nucleotides at N(−2) and N(−1). (C) Each scatter plot was fit to a line with the slope indicated in the graph. Any arrangement of points other than a line with positive slope indicates different C5 sequence specificity depending on the identity of the nucleotides at N(−1)N(−2).