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. 2017 Oct;23(10):1539–1551. doi: 10.1261/rna.061796.117

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© 2017 Dussault et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 4. — The U114:A138 long-range base pair is crucial for the formation of the F_NS state. (A) The SAM-induced folding process of the aptamer is shown with fluorescein (F) and Cy3 (Cy) dyes located on stems P1 and P4, respectively. The formation of the helical stacking is shown by a black rectangle located at the interface of both P1 and P4 stems. The curved arrow represents the helical twist that is observed upon SAM binding. (B) Folding transitions of the wild-type (black circles) and U107C mutant (white circles) aptamers as a function of the SAM concentration. The lack of significant change prevented the analysis of the U107C mutant. (C) Folding transitions of the G136C (circles) and A137U (squares) mutant aptamers as a function of the SAM concentration. (D) Folding transitions of the U114A (up-pointing triangles), A138U (circles), and U114A:A138U (down-pointing triangles) as a function of the SAM concentration. Note that U114A was not analyzed due to the lack of significant FRET change.