FIGURE 1.

Native purification of RNP complexes using the fluorescent, high-affinity Mango tool set. (A) RNA Mango aptamer core quadruplex and the GAA^A tetraloop-like connecting motif sequence (in brown, [^] indicates site of core quadruplex insertion). N and N′ are complementary base pairs of arbitrary sequence forming a RNA duplex (Trachman et al. 2017). (B) Structures of Dtb-derivatized TO1 (n = 1) or TO3 (n = 3) fluorophores. (C) General schematic for purification of native RNP complexes using Mango.