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. 2017 Oct;23(10):1592–1599. doi: 10.1261/rna.062166.117

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© 2017 Panchapakesan et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — Native purification of yeast U1 snRNP complex using RNA Mango. (A) Solution hybridization analysis of snRNAs present in native extract (NE) from U1^M tagged yeast and following Mango batch purification using TO1-Dtb (Biotin Eluate). The probed snRNAs are labeled on the right and correspond to the number labels above each lane. U1^M migrates as a doublet following RNA deproteinization and denaturation, but as a single band upon Mango-based purification. (B) Primer extension analysis to detect U1^M or U2 snRNAs following TO1-Dtb bead-based purification. (C) SDS-PAGE analysis of Snp1-SNAP_f following Mango purification.