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. 2017 Oct;23(10):1592–1599. doi: 10.1261/rna.062166.117

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© 2017 Panchapakesan et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 4. — Multiwavelength fluorescence characterization of the 6S^M:HE complex. (A) EMSA assay of the 6S^M:eGFP-HE complex visualized using eGFP-HE (green, top) and 6S^M:TO3-Dtb (red, middle) fluorescence. The bottom panel is the composite of the green and red images. Product RNA (pRNA) release was induced by the addition of NTPs and MgCl₂. (B) Fluorescence auto- and cross-correlation spectroscopy of 6S^M:TO3-Dtb (red) in the presence of 25.4 ± 0.3 nM free eGFP-HE (green) and 1% 6S^M:eGFP-HE complex (yellow, cross-correlation amplitude). (C) 6S^M:TO3-Dtb (red) in the presence of 127.5 ± 0.9 nM (right) free eGFP-HE (green) and 20% 6S^M:eGFP-HE complex (yellow, cross-correlation amplitude). Fluorescence intensity traces in thousands of photon counts per second (kcps, both red and green channels) are shown in the top panels. Residuals are shown in the middle panels. Correlation functions are shown in the bottom panels.