FIGURE 8.

CC-220 inhibits B cell proliferation and plasmablast differentiation. Naive (A) and memory (B) B cells were treated with vehicle or CC-220 (1, 10, and 100 nM) for 1 h before adding indicated stimuli and cultured for 5 d. Proliferation, plasmablast differentiation, and cell viability were assessed 5 d later by flow cytometry. Effects of CC-220 are shown as percent of vehicle control, mean ± SEM. (C) Representative plasmablast differentiation and proliferation plots for naive B cells treated with CC-220. (D) Representative plasmablast differentiation and proliferation plots for memory B cells treated with CC-220. (E) Comparison of CC-220 effects on naive and memory B cell plasmablast differentiation induced by IL-2/IL-21/CD40L. Statistics were Student t test comparison of naive and memory B cells on plasmablast differentiation at the same concentration of CC-220 treatment. (F) Comparison of CC-220 effects on memory B cell plasmablast differentiation induced by IL-2/IL-21/CD40L or IL-2/IL-21/BAFF. Statistics were Student t test comparison of CC-220 effects on plasmablast differentiation at each concentration between IL-2/IL-21/CD40L and IL-2/IL-21/BAFF treatment. (E and F) n = 6. *p < 0.05, **p < 0.005.