FIGURE 10.

CC-220 inhibits plasmablast differentiation and Ab production from DN B cells. DN B cells from HC PBMCs were sorted based on CD19⁺CD27⁻ and IgD⁻ expression, and treated with vehicle or CC-220 (10 nM) for 1 h at 37°C before adding the stimuli indicated and culturing for 5 d. (A) Data shown are the percent of plasmablasts and cell viability ± SEM relative to the total cells present after 5 d of treatment. (B) IgG (left) and IgM (right) production in the culture supernatant from DN cells were measured on day 5. Inhibition by CC-220 is shown as the percent of stimulated cells in the absence of CC-220 treatment ± SEM: IL-2/IL-21 only (black bars), IL-2/IL-21/CD40L (white bars), or IL-2/IL-21/BAFF (gray bars) (n = 5). Student t test: *p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001.