Table I. BAFF and CD40L induction of IgM and IgG secretion from naive and memory B cells with IL-2 and IL-21.
| + IL-2/IL-21 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Media | Media | Anti-IgM | CD40L | BAFF | Anti-IgM/CD40L | Anti-IgM/BAFF | BAFF/CD40L | Anti-IgM/CD40L/BAFF | |
| Naive IgM (ng/ml) | 7.7 ± 2.2 | 19.0 ± 5.3 | 22 ± 6.7 | 6306 ± 901.3*** | 135 ± 30.4* | 1996 ± 367.0** | 84 ± 18.7* | 6,225 ± 783.9*** | 2,381 ± 536.8* |
| Naive IgG (ng/ml) | 2.0 ± 1.0 | 7.7 ± 4.2 | 19 ± 7.4 | 2094 ± 505.3* | 125 ± 52.7 | 3565 ± 692.4** | 128 ± 42.8 | 2,707 ± 456.5** | 4,093 ± 692.7** |
| Memory IgM (ng/ml) | 25 ± 4.7 | 746 ± 324.0 | 345 ± 75.3 | 8595 ± 2045.3* | 3176 ± 919.3* | 4779 ± 888.7** | 1458 ± 323.3 | 11,127 ± 2215.0** | 8,343 ± 1,898.3* |
| Memory IgG (ng/ml) | 13 ± 4.3 | 2456 ± 1092.0 | 3619 ± 1286.7* | 7503 ± 1452.0** | 8465 ± 2023.7** | 7987 ± 2023.7* | 7463 ± 1734.0* | 9,783 ± 1633.0* | 11,428 ± 2,260.7** |
Naive and memory B cells were cultured under indicated conditions. IgM and IgG in culture supernatant were measured by ELISA on day 5. Numbers are mean concentration ± SEM. n = 8 for naive B cells, n = 9 for memory B cells.
*
p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001, one-way ANOVA compared with IL-2/IL-21–only condition.