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. 2017 Sep 14;11:1179069517726996. doi: 10.1177/1179069517726996

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© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Slick KO mice confirm Slick localization to neuronal subtype. (A) Illustration of Slick-LacZ global KO mice, exons 2 to 7 replaced with LacZ cassette. Right: representation of resultant β-galactosidase protein with partial Slick N-terminal. (B) Representative images of Slick KO mouse DRG X-Gal stain at low and higher magnification. Arrows indicate “punctate X-Gal” staining of the resultant β-galactosidase protein with partial Slick N-terminal. Scale bars: 20 μm. (C) Representative immunohistochemical images of Slick KO mouse DRG exhibiting no Slick (anti-mouse) immunoreactivity. Merge with DAPI and DIC. Scale bars: 20 μm. DAPI, 4,6-diamidino-2-phenylindole dihydrochloride; DIC, differential interference contrast; DRG, dorsal root ganglia; HO, knockout.