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. 2017 Jan 26;16(18):1661–1672. doi: 10.1080/15384101.2017.1281479

Figure 2.

Figure 2.

miR-34c inhibited C2C12 myoblasts proliferation. (A) After transfection 24 h, miR-34c expression was determined by qPCR in C2C12 myoblasts transfected with miR-34c mimics or negative control (NC) mimics. (B) C2C12 myoblasts were collected for cell cycle analysis. Flow cytometry was used to identify the percentage of cells in G0/G1, S, and G2 phases. (C) C2C12 cells were stained with EdU. The scale bar represents 200 μm. The percentage of EdU+ C2C12 cells was quantified (right). (D) The mRNA expression of cell cycle genes was detected by qPCR. (E) The protein expression of cell cycle genes was detected by western blotting. (F) After transfection 36 h, miR-34c expression was determined by qPCR in C2C12 myoblasts transfected with miR-34c inhibitor or NC inhibitor. (G) C2C12 myoblasts were collected for cell cycle analysis. Flow cytometry was used to determine the percentage of cells in G0/G1, S, and G2 phases. (H) C2C12 cells were stained with EdU. The scale bar represents 200 μm. The percentage of EdU+ C2C12 cells was quantified (right). (I) The mRNA expression of cell cycle genes was detected by qPCR. (J) The protein expression of cell cycle genes was detected by western blotting. All of the results are expressed as the mean ± SD *P < 0.05; **P < 0.01.