Figure 3.

miR-34c inhibited primary myoblasts proliferation. (A) After transfection 24 h, miR-34c expression was determined by qPCR in primary myoblasts transfected with miR-34c mimics or negative control (NC) mimics. (B) Primary myoblasts cells were stained with EdU. The scale bar represents 200 μm. The percentage of EdU⁺ primary myoblasts cells was quantified (right). (C) The mRNA expression of cell cycle genes was detected by qPCR. (D) The protein expression of cell cycle genes was detected by western blotting. (E) After transfection 36 h, miR-34c expression was determined by qPCR in primary myoblasts transfected with miR-34c inhibitor or NC inhibitor. (F) Primary myoblasts cells were stained with EdU. The scale bar represents 200 μm. The percentage of EdU⁺ C2C12 cells was quantified (right). (G) The protein expression of cell cycle genes was detected by western blotting. All of the results are expressed as the mean ± SD *P < 0.05; **P < 0.01.