Figure 10. Expression of Lin37 and Rb rescues the phenotype of Rb^−/− and Lin37^−/−/Rb^−/− cells.

Activities of wild-type (WT) and mutant (mutE2F, mutCHR) G₁/S (Cdc45) and G₂/M (Ttk) cell cycle gene promoters in serum-starved (A) Rb^−/− (Rb-KO) and (B) Lin37^−/−/Rb^−/− (Lin37-Rb-DKO) cells were analyzed by luciferase reporter assays upon co-transfection of plasmids expressing Rb, a Rb mutant with non-functional pocket domain (Rb-del22), Lin37, or of an empty vector (Ctrl). Mean values of relative luciferase light units (RLU) ± SD of three biological replicates are given. Significances were calculated by the Students T-Test (*p≤0.05, **p≤0.01, ***p≤0.001).