Figure 4. Knockout of Lin37 leads to a de-repression of cell cycle gene promoters in quiescence.

Wild-type (WT) or Lin37 knockout cells (Lin37-KO; clone 632–2) were transfected with luciferase reporter constructs of G₁/S (yellow) and G₂/M (blue) cell cycle gene promoters. Wild-type (wt) and DREAM-binding site deficient (E2F, CHR) promoters were tested for their activity. Lin37 knockout was rescued by re-expression of Lin37 (Lin37-pcDNA). Cells were arrested by serum deprivation. Promoter activities were normalized to Renilla luciferase activity. Mean values ± SD of three biological replicates are given, and significances were calculated by the Students T-Test (*p≤0.05, **p≤0.01, ***p≤0.001).